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                4 ίʩ

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                һίԭt

                һ\ࡢίrҎtˎᘌͬIJ^njòͬίģⷰ׷ۙͬrί壩ίS\Ͳ飻]ͬrПoԭw֧ԭwȾSTDsȾ

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                aqøPPNGqˎq_5%ϕrʹq̹ؑcxˎ

                1  ^ͿDrĘɫһ׃^3.0goΣ^c4.0goΡ

                2  ^qأ਎QܱΣ2gһ]䣬Ů4gһμ]䡣

                Zͪˎֽ̩600mgһοڷɳ200mgoΡ

                ]ZǾ͵òʧͪˎЋDcͯá

                -øƄτq^ͰSqغτ1.5gһμ],؜ c̹c,3.0gһμ]oΡ

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                ܾף^c3.0goΣɳ250mgڷһΣͷZ1g/ΣһՃɴΣ7ա

                ֱܾcף^c3.0goΣʩ۸3.0goΣξ3.0goΡ

                ģܾԨ׳ˣˮqG1000fλo}]ÿһΣ5ա

                壩ͯܲw45kg߰oˎw<45kgăͯ·oˎ^c125mgһɽ弡]䣻ʩ25mg/kgһμ]䣻^q40mg/kgһμ]䡣

                ȼ`ρKYܲ

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                ܲԭwܲpɪ١1879꣬Neisser״xܲpİʮܲpַQɪpNeisseria Gonorrhoeae1885BammѪBB@óɹ

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                Ĥ֬Ĥ׼ë²

                ëPiliԭ^㶨жμȻ˵l׃QͬľëԭԡëcƵճPͬrҲ׼ɵáB20Сr会ߡоëЂȾԨ˥rëʧӷNɸaס

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                7 вW

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                8 RF

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                һ  oρKYܲ

                һ   ԟoρKYܡ

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                ŮԟoψAKYܲ

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                кρKYܲ

                һ   кρKYܲ

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                ŮкρKYܲ

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                ʲTƬlFmp\ܲɪʲ҂˳ľȺ⌦Y͵ĉTƬԑMһz顣

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                Ļ̽\࣬õ̽У|DNA̽Ⱦɫw̽ᘺrRNA̽ᘡ

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                     ˎ|DNA̽

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                (2)Ⱦɫw̽

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                3rRNA̽

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                2ĻUzy

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                1DNAȡ

                BDNAȡ

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                RӘ˱ôȡ

                Nз2mlğoZ}ˮлPBS_ҺДDϴ1minԱ㌢˱MҺУӗȥҸҺ2-3000r/minx5minȥҺ100l 1PCR_ҺкTween 20 0.45%,øK 200g/mlҸҺ50-60ԡ1h,Ȼ95ӟ10minøK,12000 r/minx10min,ҺDNAģ塣

                2PCROؿ֮Ӌ

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                л                                              ƬLȣbp

                CPPB     NG1  5GTT TGG CTG GTT GAT TCA AG 3                 633

                         NG2  5GCA AGA TTT CCG ATTT GGC G 3

                CPPB     HO1 5GCT ACG CAT ACC CGC GTT GC 3                  390

                         HO2 5CGA AGA CCT TCG AGC AGA CA 3

                rRNA    1 5-AGG CTG TTG CCA ATA TCG GC-3                206

                        2  5-ACA CTC GAG TCA CCC AGT TC-3

                CPPB     GC1 5CTT ATC GTT TGG CTG GTT GAT TC 3              435

                         GC2 5ACC AAG ACC AAA GGT TTG ACA CTG 3

                         GC3  5ATT TTC CAG TGT CAA AC 3                      241

                         GC4  5TAT TCA AGC CCT ATC TG 3

                3PCRU

                ȡDNAȡҺ2l28lķҺУKPCRҺкdNTP100mol/L0.5mol/LTaqDNAۺø1UMg2+ 1.5mmol/L,ӟoʯϞ30l1000r/minx30sMPCRUѭhl94׃1minȻ94 30s571min721min30ѭh725min

                Ua2%ĭ֬zӾ30min廯VȾɫ¿ҊUDNAɹlӴݏɢl˳СcUеĴСһ¡ 

                4PCR`Ժخ

                CPPBڲ[|ȾɫwҲCPPBټ96%[|CPPBеИOߵ`ԣCһyһPCRGC1-GC2ԙz3ιʽPCRGC2-GC4ԙz0.39CPPB򣩡@Щ{خԌ򞣬ֻܔUDNAɋɪϾUخa

                5ιܳʽPCR

                ڂyʽPCRĻAόɌPCROӋޣʽȃɂGC1GC225b˻ض^ߣ68棩ʽȂȃɡтGC3-GC417b˻ض^ͣ46棩PCRҺġɷcһPCRͬ@ӣͨ^˻ضȣ68棩ʹeДU^20-30ѭhᣨһPCRٽ˻ضȣ46棩ʹԵڳDZʲô˼һPCRaģMгʽUԓPCR`ȿˮԪϿ_z0.3

                6Bø朷LCPzy

                ĿǰPCRzyķVʹáخԣ`ԲկsfdzƽoߡͬrһN\༼gBø朷LCPҲ•خ`ԱęzyСLCP cPCR֮ͬ̎LCP ĢٌøBøBøԌɗlBBăɗlɗlơģ壬BøСBӣֿģ壬M30-40ѭhLCPõģ̎cLPCRģƨȡLCPõ̽ᘳһ˿OӋCPPBϣҲOӋȾɫwϣopa-1Abbottopa-148bpLą^OӋ4LCP̽ᘣopa-1Ⱦɫw11؏͡ˣԓMLCP̽ᘾи`ԺخԡLCP^̣

                ģLCPҺУLCPҺ20mmol/L  Tris-HCl pH7.6;100 mmol/L KCl 10 mmol/L MgCl21 mmol/L EDTA; 10 mmol/L NAD+;10 mmol/L DTT,Иӛăɂ̽ᘸ40fmol/Lδӛ̽ᘸ40fmol/L15U͟Bøl971s551s6250s40ѭh100laø˰΢УM@ɫø˃xxȡֵBuimerČCLCPڙzyӘ˱`Ȟ100%Һ˱88.9%ŮԌmiӘ˱95.4%LCP{ɫخԸ_100%@һc@PCRخԣ˼Եİl

                3 R\]헡

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                1OӋ ȻlеPCR⣬߀ɏOӋ

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                2R˱̎ R󲿷ɄtھLɽ˱vPCRģҪԽԽá@Ҫڲɼ˱rҪȡƽͻȻFһӰ_λã춟oY˸ҵǧ ҪmɘԱCɼIԭwR˱ɷֱ^sЕr̎Ę˱PCRUΡЧK@s|^ɵ,ҪMһ`ӡ÷һȷ³YD@N^韩,ĿǰƷDNAԇ,^؏R˱ȡ߼ȵDNA

                3PCRaęzy  һΛ]кƳǰRPCRzy׺ӾķMPCRabԓS}^^ԶɼԺĽYĿǰs@ɫӾ˽YДخԺ` ֪ԡ

                ֮PCRcLCPȂyB`Ժخ˺ܡߣrgҲs̡S\༼gIJMPCRcLCPĩ΢΢ęzyɞ鳣Ҏęzyһ

                壩ˎԇ򞣺BMһˎԇ򞡣üƬUɢԇ򞣬í֬ƽϡጷyóһZ耳׶С־MICָxÿء

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